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Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795) Specific References (38) Description: Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285) Specific References (39) Description: FITC Conjugation Kit (Fast) - Lightning-Link . FAQs for Lightning-Link (R) Rapid Alexa Fluor 647 Kit (336-0030). Format: Alexa Fluor 700 (A=675-700, E=723) Kit Type: Antibody Labeling Kit. Lightning-Link (R) Rapid Alexa Fluor 700 Isotype Control [Alexa Fluor® 647] [337-0015] - Scans of the Absorbance and Fluorescence Emission of a Lightning-Link® Rapid Alexa Fluor® 700 Conjugate . Molecular Probes™ Alexa Fluor™ 488 Protein Labeling Kit. Alexa Fluor™ 647 Microscale Protein Labeling Kit (Invitrogen™) And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved . Learn More. The SAIVI™ Alexa Fluor™ 647 Antibody/Protein 1 mg-Labeling Kit provides a convenient means to label proteins with Alexa Fluor™ 647 near-IR emitting dye (Figure 1). Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. Lightning-Link® Rapid Alexa Fluor 647 Antibody Labeling Kit Filamentous actin was labeled with . Molecular Probes™ Alexa Fluor™ 647 Antibody Labeling Kit 1 ... Extinction coefficient: 24,000. BP Fluor 647 is water soluble and pH-insensitive from pH 4 to pH 10. BP Fluor 647 Mal Antibody Labeling Kit (1 x 1 mg) | BroadPharm Description: Molecular Probes Protein Labeling Kits provide a convenient means for attaching a fluorescent label or biotin to an antibody or a protein larger than 40 kDa Conjugates are ideal for multiple applications including flow cytometry fluorescent microscopy immunohistochemistry primary . Name: Alexa Fluor 647 Protein Labeling Kit. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Wilms Tumor Protein antibody [EPR23963-116] (ab283325) Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Wilms Tumor Protein with abab283325 at 1/100 (5.0 ug/ml) followed by a at dilution. Alexa Fluor® 790 A20189 Protein Labeling Kit—Complete, easy-to-use kit designed for three reactions, each reaction optimized to label ~1 mg of protein. Note 2: . Alexa Fluor 647 dye | Thermo Fisher Scientific - DE Alexa Fluor™ 647 Microscale Protein Labeling Kit Lightning-Link® Rapid Alexa Fluor 647 Antibody Labeling Kit Summary. Completely dissolve sodium bicarbonate in 1 mL of MilliQ water by vortexing or pipetting to make 1 M sodium bicarbonate solution. Fluorophore label: Alexa Fluor™ 647 dye Reactive group: maleimide Reactivity: thiol groups on proteins and ligands, oligonucleotide thiophosphates Ex/Em of the conjugate: 651/671 nm Extinction coefficient: 265,000 cm-1M-1 Spectrally similar dyes: Cy5 Molecular weight: ∼1250 Typical Conjugation Reaction The protein should be dissolved at a concentration of 50-100μM in a suitable buffer (10 . Alexa Fluor®, Pacific Blue™, and Pacific Orange™ Antibody Labeling Kits | 2 Alexa Fluor® dyes produce protein conjugates that are typically brighter, more photostable, and less pH sensitive relative to the traditional dyes (AMCA, fluorescein/FITC, TRITC, etc.) Alexa Fluor 647 Protein Labeling Kit from Thermo Fisher Scientific. Protein Labeling Products | NEB 1). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence . The SAIVI Alexa Fluor 647 Antibody/Protein Labeling Kit provides the materials needed conveniently label an antibody or protein with amine-reactive far-red fluorescent Alexa Fluor 647 dye (ex/em 650/665 nm) for in vivo animal imaging applications. The dye is water soluble and pH-insensitive from pH 4 to pH 10. Alexa Fluor 647 Protein Labeling Kit from Thermo Fisher ... About Biocompare About . Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Pricing and Availability. Molecular Probes Alexa Fluor ® 633 Protein Labeling Kit pro-vides a convenient means to label proteins with our superior Alexa Fluor 633 dye. . Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines..Alexa Fluor 594 protein/antibody labeling kits. A 405 nm-excitable dye with orange fluorescence emission. A significant advantage of long wavelength dyes such as Alexa Fluor® 647 is the low autofluorescence of biological specimens in this region of the spectrum. The purified protein was conjugated with Alexa Fluor® 647 under optimal conditions. A20173 $528.00 / Each Qty Add to cart Description Specifications Safety and Handling Description Molecular Probes™ Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). HiLyte Fluor* 647 Labeling Kit-NH 2 is mainly used for the preparation of red fluorescence-labeled proteins, such as IgG, for immunostaining, and cellular proteins for tracing. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. These three dyes, BP Fluor 647, Cyanine . Menu Sign in or Register Search; Custom Suppliers; Data; Citations; Images; Listing; Contact ; 2022 CiteAb Awards - Nominate now for the 2022 CiteAb Environmental Initiative of the Year Award! Thank you! 332-0015 is the same as the 1 mg size. The Alexa Fluor® 488 reac-tive dye (Figure 1) has a TFP ester moiety that is more stable in solution than the commonly used succinimidyl (NHS) ester. we have the lightning link rapid alexa fluor 647 Antibody labeling kit and i was wondering if we can use it with IgG1 that contains 0.09% sodium azide phosphate-buffered solution. However, because of its peak emission at 667 nm, Alexa Fluor . 3. * 1. Mouse intestine section labeled with four fl uorescent dyes. Quick Protocol. Alexa Fluor 647 Protein Labeling Kit. Learn about our antibody labeling kits and their advantages. Contact ; Technical . Lightning-Link Rapid Antibody Labeling kits enable direct labeling of antibodies, proteins, peptides or other biomolecules (see protocol for further information) and allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes). From start to finish, it takes less than three hours to label a protein and purify the resulting conjugate using the Alexa Fluor® 647 Protein Labeling Kit . The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the . Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several bonds. Description: Molecular Probes Protein Labeling Kits provide a convenient means for attaching a fluorescent label or biotin to an antibody or a protein larger than 40 kDa Conjugates are ideal for multiple applications including flow cytometry fluorescent microscopy immunohistochemistry primary detection ELISAs immunocytochemistry FISH and more Kits are . BP Fluor 647 (Alexa Fluor 647 ® equivalent) is a far-red bright and photostable fluorophore absorbed at 650 nm and emitted at 671 nm. Order Details. SNAP-tag ® Purified Protein. protein conjugate solution need be concentrated or freeze dried. Important Features of Alexa Fluor™ 647 Antibody Labeling Kit: With an excitation and emission maximum of 650/668 nm, Alexa Fluor™ 647 can be efficiently excited using a 633 or 635 nm Kr or He-Ne laser line and detected under standard APC/Cy5™ filters Labeled proteins typically ready to use typically in 90 min (~15 min hands-on time) PEG spacers increase water solubility, enable efficient biolabeling in imaging and diagnostic R&D. Alexa Fluor dyes, Bodipy, Cyanine, Fluorescein, Rhodamine and Pyrene dyes. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. The SAIVI™ Alexa Fluor® 647 Antibody/Protein 1 mg-Labeling Kit provides a convenient means to label proteins with Alexa Fluor® 647 near-IR emitting dye (Figure 1). The Alexa Fluor® 488 Protein Labeling Kit contains everything that is needed to perform three separate labeling reactions and to purify the resulting conjugates. ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). 2. SNAP-tag ® Purified Protein. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. CF®405L Dye. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in transfected cells. Generate ready-to-use, Alexa Fluor-labeled antibodies even in the presence of contaminants with APEX Alexa Fluor Antibody Labeling kits. Fluorescent dye is a fluorescent chemical compound that can re-emit light upon light excitation. ized with an Alexa Fluor 647 goat anti-chicken IgG (pseudocolored purple), goblet cells were labeled with an Alexa Fluor 594 conjugate of wheat germ agglutinin (red), and nuclei were labeled with anti-cdc6 peptide antibody and visualized with an Alexa Fluor 405 goat anti-mouse IgG (blue). This kit is optimized for labeling 1 mg of IgG antibody per reaction with Alexa Fluor 647. Alexa Fluor® 647 Conjugation Kit / Alexa Fluor® 647 Labeling Kit ab269823 uses a simple and quick process for Alexa Fluor ® 647 labeling / conjugation of antibodies. Product Description. In the Microscale Protein Labeling Kits, the reactive dye contains a succinimidyl (NHS) ester moiety that reacts with primary amines of proteins to form stable dye-protein conjugates. Molecular weight: ~1573. TFP esters react efficiently with primary amines of proteins to form stable dye . Protein Labeling Kits (For Alexa Fluor . Lightning-Link® Rapid Alexa Fluor 647 Antibody Labeling Kit Summary Applications/Dilutions Application Notes By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. Alexa Fluor 647 is conjugated to NT-Lys using Alexa Fluor 647 protein labeling kit targeted to primary amines, following the manual of the manufacturer. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. 10 ug) and large quantities of primary antibodies with ease. Prepare a 1 M sodium bicarbonate solution by resuspending Component B in 1 mL of deionized water. So far in vitro transcription-based direct labeling of RNA probes with AF (Alexa Fluor®)-Dyes is rather inefficient.. We now offer HighYield T7 RNA Labeling Kits for efficient AF (Alexa Fluor®)-Dye labeling.. AF (Alexa Fluor®)-Dyes are hydrophilic fluorescent dyes with remarkable photostability however, when conjugated to normal 5-UTP, they show rather poor incorporation (Fig. Sign in or register to save this reagent to your favourites Search (Z-25108) Zenon™ Alexa Fluor™ 647 Mouse . Alexa Fluor 647 dye dashboard Important Features of Microscale Protein Labeling Kits: The dye has 4 sulfonate groups make it high water soluble and less aggregation in the aqueous solution. Microscale Protein Labeling Kit— Complete, easy-to-use kit designed for three reactions, each reaction optimized to label ~20-100 µg of protein. The sulfonate groups make it high water soluble and less aggregation in the aqueous solution. What does Alexa Fluor 594? The Alexa Fluor 647 Protein Labeling Kit provides the materials needed to conveniently label an antibody or protein with amine-reactive far-red fluorescent Alexa Fluor 647 dye (ex/em 650/665 nm). It can also be used to conjugate other proteins or peptides. 332-0010 is the same as the 3 x 100 ug size. I was extremely impressed with this kit, as it is very well designed and easy to use. Add the . Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20-100μg). (A-20186) Alexa Fluor™ 647 Antibody Labeling Kit - Thermo Scientific - CiteAb. Kit [A-20186] Alexa Fluor™ 647 Antibody Labeling Kit by Invitrogen Antibodies. Unlike other dyes, the fluorescence of Alexa Fluor®-conjugates is insensitive to pH between pH 4 . Alexa Fluor™ 488 Protein Labeling Kit. Description: Molecular Probes Protein Labeling Kits provide a convenient means for attaching a fluorescent label or biotin to an antibody or a protein larger than 40 kDa Conjugates are ideal for multiple applications including flow cytometry fluorescent microscopy immunohistochemistry primary detection ELISAs immunocytochemistry FISH and more Kits are . ReadiLink™ Rapid XFD647 Antibody Labeling Kit *XFD647 Same Structure to Alexa Fluor™ 647* Author: AAT Bioquest Subject: Protocol Sheet for Catalog No. Generate antibody conjugates for fluorescent microscopy, flow cytometry, IHC, ICC, and more in just 15 minutes with Zip Alexa Fluor Rapid Antibody Labeling kits. Find ALEXA FLUOR ™- 647 Protein Labeling Kit and related products for scientific research at MilliporeSigma Wish list; My account ; Shopping cart 0 0.00€ * Service/Help . Show more Hide. Technical Summary. Comparable amounts of other proteins (>30 kDa) can also be labeled. ized with an Alexa Fluor 647 goat anti-chicken IgG (pseudocolored purple), goblet cells were labeled with an Alexa Fluor 594 conjugate of wheat germ agglutinin (red), and nuclei were labeled with anti-cdc6 peptide antibody and visualized with an Alexa Fluor 405 goat anti-mouse IgG (blue). In the Microscale Protein Labeling Kits, the reactive dye contains a succinimidyl (NHS) ester moiety that reacts with primary amines of proteins to form stable dye-protein conjugates. Kit [Z-25108] Zenon™ Alexa Fluor™ 647 Mouse IgG2a Labeling Kit by Invitrogen Antibodies. The kit is designed for labeling and purifying 1 mg of protein per reaction, and has been optimized using 1 mg of IgG per conjugation reaction. The Alexa Fluor 647 Monoclonal Antibody Labeling Kit provides a convenient means to label small amounts of monoclonal antibodies with the superior Alexa Fluor. Alexa Fluor® 647 conjugates are the best choice of far-red emitting dyes for multiple labeling detection with a confocal microscope, and are also widely used in flow cytometry. Concentration Lot . A major advantage of Mix-n-Stain™ kits is our superior CF® dyes, many of which are brighter and more photostable than Alexa Fluor® dyes and other similar commercial dyes. Add 50 μL of the three vials of reactive dye has a succinimidyl ester is... Have minimal… EN //www.jacksonimmuno.com/technical/products/conjugate-selection/alexa-fluor/647 '' > Alexa Fluor 647? < /a > SNAP-tag ® purified.! [ A-20186 ] Alexa Fluor™ dyes are brighter, more photostable, and alexa fluor 647 protein labeling kit... Necessary for labeling proteins, in particular, antibodies IgG antibody per reaction with Alexa 647... To traditional dyes, bp Fluor 647 is water soluble and pH-insensitive from pH and. With this kit, as it is very well designed and easy to use 4 sulfonate groups make high. 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