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Mouse List KO&KI PDF Alexa Fluor® 488 Protein Labeling Kit Kits are available in 12 Alexa Fluor™ dye colors, biotin, the hapten Oregon Green™ 488, fluorescein EX, and Texas Red™ dye. EdU | 5 EdU detection Incorporated EdU can be detected with a Click-iT® EdU Kit (Table 3) or with an available dye- or hapten-containing azide (Table 4). This kit is optimized for labeling 100 µg of antibody per reaction with green fluorescent Alexa Fluor® 488. It can also be used to conjugate other proteins or peptides. PRODUCT INFORMATION SHEET Version: 1.0 Revision Date: 08/08/2020 BP Fluor 488 Antibody Labeling Kit Components Overview BP Fluor 488 (Alexa Fluor® 488 equivalent) is a green fluorescent dye for the 488 nm argon laser line. Lightning-Link® Rapid Alexa Fluor 700 Antibody Labeling Kit Molecular Probes™ Alexa Fluor™ 594 Protein Labeling Kit 1 ... Learn More About Protein and Antibody Labeling We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. HA also mediates membrane fusion between the virus and a cell, which is the first critical step during infection. Each kit provides the components needed to perform three protein conjugations and purifications. Quick Protocol. The kit includes everything required for the labeling, so you only need unlabeled target protein/antibody. Alexa Fluor®-594 FluoroNanogold™ Fab' goat anti guinea pig IgG (H+L) Alexa Fluor®-594 FluoroNanogold™ -Fab' conjugates: Instructions and Protocols: SDS : 7554-1ML 7554-0.5ML: Alexa Fluor® 647 - FluoroNanogold™ IgG goat anti guinea pig IgG (H+L) Instructions and Protocols: SDS : 7555-1ML 75555-0.5ML View our protocol for Staining Membrane-associated Proteins. improving abcam.com and welcome your feedback. Primary antibodies Secondary antibodies ELISA and Matched Antibody Pair Kits Cell and tissue imaging tools Cellular and biochemical assays Proteins and Peptides By product type Proteomics tools Agonists, activators, antagonists and inhibitors Cell lines and Lysates Multiplex miRNA assays Multiplex Assays By research area Cancer Cardiovascular . This kit is optimized for labeling 100 µg of antibody per reaction with far red fluorescent Alexa Fluor® 647. To conjugate an antibody to Alexa Fluor ® 647 using this kit: - add modifier to antibody and incubate for 15 mins - add quencher and incubate for 5 mins The Alexa Fluor ® 647 conjugated antibody can be used immediately in WB, ELISA, IHC etc. Alexa Fluor® 488 Protein Labeling Kit | 4 3.3 Calculate the degree of labeling: moles dye per mole protein = A 494 × dilution factor where 71,000 cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® 488 dye at 494 nm. Prepare Ab>1mg/ml in PBS . Molecular Probes® Alexa Fluor® Antibody Labeling Kits provide a convenient means to label small amounts of antibodies with Alexa Fluor® dyes (choice of 10 colors). Two of the fluorescent dyes, Alexa Fluor® 488 and Oregon Green® 488 can be used as fluorescent dyes or haptens with an anti-dye antibody. No. Excitation (nm) Emission (nm) Application Alexa Fluor® 488 A10468 496 519 Fluorescent label for use in imaging or flow . Donkey Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (Thermo Fisher Scientific Cat# A-21202) was used at 1:300 for immunofluorescence. Invitrogen™ Alexa Fluor 594 dye is a bright, red-fluorescent dye with excitation ideally suited to the 594 nm laser line. The addition of the ALEXA Fluor 488 label to Lightning-Link kits now enables easy, direct conjugation of the label to primary antibodies. Monoclonal Antibody for studying E-Cadherin in the research area. kit is sufficient for labeling ~1 mg of an IgG antibody, although other proteins can also be labeled. Lightning-Link Rapid Antibody Labeling kits enable direct labeling of antibodies, proteins, peptides or other biomolecules (see protocol for further information) and allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes). Kaninchen Polyklonal SIRT6 Antikörper AA 290-319 für IHC, ELISA, WB. We got a very nice conjugated antibody band in the resin. Amount and volume of antibody for conjugation to Alexa Fluor ® 568 Lightning-Link® Rapid Alexa Fluor 700 Antibody Labeling Kit Summary. 031-728-3233 E-mail. 10 ug) and large quantities of primary antibodies with ease. Lightning-Link® Rapid Alexa Fluor 647 Antibody Labeling Kit Summary Applications/Dilutions Application Notes By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. After collecting the elute, we tried to measure the . Readilink(TM) protein labeling technology is the most robust and convenient tool for preparing fluorescent antibody conjugates used for fluorescence… These images indicate that the Alexa Fluor ™ 488 Tyramide SuperBoost Kit is more sensitive than both the TSA ™ kits and directly labeled secondary . Table 2. APEX™ antibody labeling tip. Flow cytometric analysis can be performed without any further staining of the cells. Finger Stick Blood Test to Assess Post Vaccination SARS-CoV-2 Neutralizing Antibody Response Against Variants. We recently used this kit to label some purified antibodies that were made in the lab for flow cytometry. To conjugate an antibody to Alexa Fluor ® 488 using this kit: Anti-CaMKII antibody [EP1829Y] (Alexa Fluor . Alexa Fluor®, Pacific Blue™, and Pacific Orange™ Antibody Labeling Kits | 3 Before You Begin Preparing the Protein IMPORTANT: The purified protein must be in a buffer free of ammonium ions or primary amines, as they will compete with the amine groups of the protein for the reactive dye. Buy ReadiLink(TM) Rapid AF488 Antibody Labeling Kit *Rapid Alexa Fluor 488 Labeling*, item number: ABD-1275 from AAT Bioquest at Biomol! Lightning-Link® Rapid Alexa Fluor 568 Antibody Labeling Kit Summary Description Lightning-Link Rapid Antibody Labeling kits enable direct labeling of antibodies, proteins, peptides or other biomolecules (see protocol for further information) and allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes). Solutions and Reagents. Fluorescent labeling of Alexa Fluor® 647 and Nanogold® - Fab' tertiary probe. Take look. The new Alexa dyes include: 647, 700, 555, 568, and 694. Here is the list of knock-out (KO) and knock-in (KI) mouse lines generated by gene targeting or genome editing at LARGE as collaborations with investigators at BDR and a number of other academic institutions. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA . Alexa Fluor® 594 Conjugation Kit / Alexa Fluor® 594 Labeling Kit (ab269822) uses a simple and quick process for Alexa Fluor ® 594 labeling / conjugation of antibodies. Streptavidin Alexa Fluor ® 594 Monovalent Antibody Labeling Kit ab272189 is used to label biotinylated antibodies with Alexa Fluor ® 594.. technical@dawinbio.com 카카오톡 플러스친구 ID. antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins. Enables easy, fast labeling of primary antibodies or proteins with ALEXA Fluor 488 Innova Biosciences (Innova), a specialist provider of bioconjugation products and services, has announced it has added ALEXA Fluor® 488, one of the most popular fluorescent labels used by life science researchers, to its Lightning-Link® range of antibody and protein labeling kits.. Antibody Labeling Kits. Lightning-Link allows for conjugation of antibodies and proteins in less than 20 minutes, using a very simple protocol which is free of separation steps, saving researchers time and precious materials. For labeling smaller amounts of proteins (~100 µg), we recommend our Alexa Fluor 555 Monoclonal Antibody Labeling Kit (A20187). Description. Please follow the "Detail" link for further information. The Antibody - Streptavidin Alexa Fluor ® 594 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes.. Several antibodies can be labeled with this kit, and its associated kits, and . For details see Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum—Section 1.3. The Antibody - Streptavidin Alexa Fluor ® 488 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes. The Antibody - Streptavidin Alexa Fluor ® 594 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes.. Several antibodies can be labeled with this kit, and its associated kits, and . Two of the most important developments in fluorescence microscopy over the past one to two decades are super-resolution microscopy, for imaging small features beneath the ~250-nm diffraction limit of visible light, and cleared tissue microscopy, for deep imaging of intact specimens (1-5).Researchers are now able to routinely measure nanoscale molecule distributions, protein . Zip Rapid Antibody Labeling Kits Zip Alexa Fluor Rapid Antibody Labeling Kits allow you to efficiently label your precious antibody with fluorescent dyes to create an antibody conjugate that is ready to use within 15 minutes. ReadiLink™ Rapid iFluor™ 488 Antibody Labeling Kit (AAT at#1255) vs. Lightning-Link® Rapid Alexa Fluor® 488 CD4(SK3)-488 conjugate Stain Index . As specialists in the flow cytometry field, our antibody portfolio includes an extensive selection of primary antibodies tested in flow cytometry, including our new StarBright Dye conjugated antibodies. Monoclonal antibodies are often available only in small quantities and this kit is optimized for labeling 100 µg of antibodies per reaction. Images were taken and analyzed on EVOS ™ FL Auto Imaging System (Cat. Rabbit Polyclonal MAFA antibody AA 203-231 for ELISA, WB. modern browser such Google Chrome Hello. 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